Using a mouse model system for our initial studies, we have demonstrated that the alginate hydrogel-based follicle culture system can support follicle growth from very early follicular stages. Fully-grown oocytes derived from this follicle culture system can mature into metaphase II-arrested eggs that are capable of being fertilized and giving rise to live pups. We have also demonstrated that the alginate hydrogels can support follicle growth following cryopreservation, which is clinically significant.

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A Novel Two-Step Strategy For In Vitro Culture Of Early-Stage Ovarian Follicles In The Mouse Research Resources, Basic Science, Mouse Follicle Culture, Oncofertility-Related Publications Download
Regulation of Mouse Follicle Development by Follicle-Stimulating Hormone in a Three-Dimensional In Vitro Culture System Is Dependent on Follicle Stage and Dose Research Resources, Basic Science, Mouse Follicle Culture, Oncofertility-Related Publications Download
Secondary Follicle Growth and Oocyte Maturation by Culture in Alginate Hydrogel Following Cryopreservation of the Ovary or Individual Follicles Research Resources, Basic Science, Mouse Follicle Culture, Oncofertility-Related Publications Download
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