Oncofertility Consortium members, Ting AY, Yeoman RR, Campos JR, Lawson MS, Mullen SF, Fahy GM, Zelinski MB, published a paper in Human Reproduction entitled, Morphological and functional preservation of pre-antral follicles after vitrification of macaque ovarian tissue in a closed system. Researchers asked the question: what are the appropriate conditions to vitrify the macaque ovarian cortex in a large-volume, closed system that will preservefunctional pre-antral follicles? They determined the combination of glycerol, ethylene glycol (EG) and polymers with cooling in liquid nitrogen (LN(2)) vapor and a two-step warming procedure was able to preserve tissue and follicle morphology as well as function of a small population of secondary follicles in themacaque ovarian cortex following vitrification in a closed system. Read Morphological and functional preservation of pre-antral follicles after vitrification of macaque ovarian tissue in a closed system.
