3D movie generated from a 100μm confocal z-stack (Zeiss LSM 510, 40x) of a human, 14-day in vitro growth follicle. This movie shows the newly formed antral cavity, the differentiation of a clear cumulus cell mass around the oocyte, and the mural granulosa cell layers. [F-Actin (red), Nuclei (blue)]. Movies were made on Volocity (Improvision/PerkinElmer).
SUPPLEMENTARY TEXT:
3-dimensional movie of confocal reconstructions, taken by Susan Barrett, PhD